Rapid and Sensitive Determination of Polycyclic Aromatic Hydrocarbons in Green Tea Samples by Dispersive Liquid-Liquid Microextraction Coupled to High-Performance Liquid Chromatography-Diode Array Detection

A rapid and sensitive dispersive liquid-liquid microextraction (DLLME) has been developed for the extraction of selected polycyclic aromatic hydrocarbons (PAHs), namely phenanthrene (PHE) fluoranthene (FLA) in green tea beverage samples. The extracted PAHs were separated determined by using high-performance liquid chromatography diode array detection (HPLC-DAD). An experimental design response surface methodology (RSM) central composite (CCD) was performed to evaluate interactive effects 2 most significant parameters, time sample pH. optimal conditions pH 6.5 with 1.25 min time. analysis variance (ANOVA) showed that influential parameter predicted values obtained good agreement actual value (R2 ≥ 0.9086). Under conditions, method demonstrated acceptable linearity 0.9996) over a concentration range (10 - 1,000 µg L−1) different PAHs. limits quantifications (4 10 (18 50 L−1), respectively. Good analytes recovery (102 110 %) excellent precision level relative standard deviations (RSD %, n = 3) between 1.53 4.52 %. successfully applied spiked proposed is rapid, reliable, sensitive, environmentally friendly PAHs.
 HIGHLIGHTS
 
 more efficient preparation required measure interferences enrich target residues tea, as existing methods are time-consuming, tendency form emulsions, consume large volumes solvents, involve disposal toxic or flammable chemicals
 Dispersive coupled detector (HPLC-DAD) selective PAHs, samples
 RSM optimized DLLME parameters based on CCD, used during PHE FLA samples non-polar sensitivity. procedure simple, minimized consumption organic solvent
 GRAPHICAL ABSTRACT